Differential expression of delayed rectifier potassium channel subtypes in rat smooth muscles.

functional expression of delayed rectifier potassium channels in cardiomyocytes ‎derivedfromembryonicstemcells

Cloning and expression of a Kv1.2 class delayed rectifier K+ channel from canine colonic smooth muscle.

A cDNA (CSMK1) encoding a delayed rectifier K+ channel of the Kv1.2 class was cloned from canine colonic circular smooth muscle and expressed in Xenopus oocytes. These channels appear to be uniquely expressed in gastrointestinal muscles and may participate in the electrical slow wave activity. Functional expression of CSMK1 in Xenopus oocytes demonstrated a K+ current that activated in a voltag...

Molecular identification of a component of delayed rectifier current in gastrointestinal smooth muscles.

Kv2.2, homologous to the shab family of Drosophila voltage-gated K+ channels, was isolated from human and canine colonic circular smooth muscle-derived mRNA. Northern hybridization analysis performed on RNA prepared from tissues and RT-PCR performed on RNA isolated from dispersed and selected smooth muscle cells demonstrate that Kv2.2 is expressed in smooth muscle cells found in all regions of ...

Differential expression and alternative splicing of TRP channel genes in smooth muscles.

Nonselective cation channels (NSCC) are targets of excitatory agonists in smooth muscle, representing the nonselective cation current I(cat). Na(+) influx through NSCC causes depolarizations and activates voltage-dependent Ca(2+) channels, resulting in contraction. The molecular identity of I(cat) in smooth muscle has not been elucidated; however, products of the transient receptor potential (T...

Contribution of delayed rectifier potassium currents to the electrical activity of murine colonic smooth muscle.

1. We used intracellular microelectrodes to record the membrane potential (Vm) of intact murine colonic smooth muscle. Electrical activity consisted of spike complexes separated by quiescent periods (Vm approximately -60 mV). The spike complexes consisted of about a dozen action potentials of approximately 30 mV amplitude. Tetraethylammonium (TEA, 1-10 mM) had little effect on the quiescent per...